Two methods were used to measure the specific activity (in units of enzyme activ

Question

Two methods were used to measure the specific activity (in units of enzyme activity per milligram of protein) of an enzyme. One unit of enzyme activity is defined as the amount of enzyme that catalyzes the formation of one micromole of product per minute under specified conditions Find the mean, standard deviation, and % relative standard deviation for each set It's fine to do the mean and standard deviation on your calculator and simply report the result, please show your %rsd calculation Calculate the 95% confidence interval for each data set. In a complete sentence, and using method 1 as an example, explain what is represented by the standard deviation, and what is represented by the 95% confidence interval Do the methods have significantly different precisions? Support statistical test, at the 95% confidence level

Explanation / Answer

Method 1

Mean = 148.8

Std. Dev. = 10.3295

Relative Std. Dev. = 100 * 10.3295 / 148.8 = 6.9418

Method 2

Mean = 154.7143

Std. Dev = 6.7259

Relative Std. Dev. = 100 * 6.7259 / 154.7143 = 4.3473

Confidence Intervals

Method 1

Lower Limit = 148.8 - t.95, n-1 * (Std.Dev./sqrt(n)) = 135.974146

Upper Limit = 148.8 + t.95, n-1 * (Std.Dev./sqrt(n)) = 161.625854

Method 2

Lower Limit = 154.7143 - t.95, n-1 * (Std.Dev./sqrt(n)) = 146.362944

Upper Limit = 154.7143 + t.95, n-1 * (Std.Dev./sqrt(n)) = 163.065627

Standard deviation refers to the range of separation from the mean in which actual data will tend to fall in, while the confidence interval is that in which with a 95% of confidence, present and future data will fall within, assuming a t student distribution behavior of the sample, as we can see, the upper limit from the confidence interval does not belong to the data present in the table.

I'll  be glad to answer the remaining question and more doubts related to this or any other question, just be sure to post them in a different question.

1 144 147 160 135 158 2 148 159 156 164 159 145 152

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.