Use the following table below to answer this question. How many complementation

Question

Use the following table below to answer this question. How many complementation groups are there and which mutants belong to the same complementation groups? You have identified some intermediates in threonine synthesis: A, B, C, D and E. You grow a few of your mutants in the presence of these different intermediates to determine the order in which the gene products act. Below are your results. A means growth and a means no growth. Given the data below, draw the best possible pathway for the synthesis of threonine. The diagram should use arrows to indicate one intermediate being changed to another intermediate. Indicate which gene produces the product responsible for the conversion by listing the mutant in that gene above the arrow. For example, if the gene product that is mutant in mt8 is responsible for converting A to B that would be drawn as:

Explanation / Answer

35) There are 4 complementation groups.

This is identified by looking at each pair of mutants and the complementation between them. In the matrix given in the question '+' indicates growth and '-' indicates no growth. In the matrix, we can find that combination of mt1 and mt2 is able to grow and they complement each other and belong to one complementation group. In the next row, we see that mt3 and mt1 fail to complement, therefore they are in the same gene. However, mt3 and mt2 complement each other, so they are in different complementation groups. Continuing down the matrix like this, we arrive at 4 complementation groups. The mutants belonging to each complementation group is given below,

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