Although quite inefficient, cloning by nuclear transplantation can be successful

Question

Although quite inefficient, cloning by nuclear transplantation can be successful, as exemplified by cloning of the famous Dolly. Such a success implies that … A.nuclei can be completely reprogrammed by cytoplasmic factors in a foreign cytoplasm. B.epigenetic changes in somatic cells are functionally irreversible. C.even a differentiated nucleus contains a complete genome, capable of supporting the development of an entire organism. D.All of the above. Although quite inefficient, cloning by nuclear transplantation can be successful, as exemplified by cloning of the famous Dolly. Such a success implies that … A.nuclei can be completely reprogrammed by cytoplasmic factors in a foreign cytoplasm. B.epigenetic changes in somatic cells are functionally irreversible. C.even a differentiated nucleus contains a complete genome, capable of supporting the development of an entire organism. D.All of the above. Although quite inefficient, cloning by nuclear transplantation can be successful, as exemplified by cloning of the famous Dolly. Such a success implies that … A.nuclei can be completely reprogrammed by cytoplasmic factors in a foreign cytoplasm. B.epigenetic changes in somatic cells are functionally irreversible. C.even a differentiated nucleus contains a complete genome, capable of supporting the development of an entire organism. D.All of the above.

Explanation / Answer

During cloning of the famous Dolly, various attempts were undertaken to improve the efficieny of nuclear transfer. The donor cells and recipient cells were synchronized, somatic cells from donors of various ages were examined under various culture conditions and epigenetic markers of donor cells were modified by drugs. Formation of a new organism proved that nuclei could be completely reprogrammed by cytoplasmic factors in a foreign cytoplasm.

Answer: (A)

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