the use of TLC to monitor the reaction of vanillin with enzymes produced by yeas
ID: 902585 • Letter: T
Question
the use of TLC to monitor the reaction of vanillin with enzymes produced by yeast relies on the on the fact that the reactant and product are expected to have very different Rf values. Could you use the same approach to monitor other reactions in which the reactant and product have very different Rf values? Explain concept clearly, using diagrams to illustrate what you would expect to observe during a TLC analysis (as the reaction progresses toward completion) when the product is much LESS polar than the reactant
Explanation / Answer
putthe spots of reactants and reaction mixture on a tlc just abot 1 cm abovethe TLC plate bottom
run the tlc in some low polarity solvent and chek the spots.If product is formed a spot will apear above reactant spot.
you can run the tlc many times with increasing polarity solvents
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