Suppose that you carry out the experiment shown in the table below. You use the

Question

Suppose that you carry out the experiment shown in the table below. You use the Nelson’s Assay to measure reducing sugars as was done during the starch lab. Note that a % solution means g/100 mL, so a 1% starch solution has 1000 mg/100 mL = 10 mg/mL. Mass of bean leaves = 3g Dilution Tested % Transmittance read for given dilution mL of DI-H2O used for grinding = 30 mL No dilution %T = 9%, mL of diastase added = 3 mL 1/5dilution %T = 50%, Volume of filtrate = 33 mL 1/20dilution %T = 91%. Find mg starch per 1g fresh weight.

Explanation / Answer

Note that a % solution means g/100 mL,

so a 1% starch solution has 1000 mg/100 mL = 10 mg/mL.

Mass of bean leaves = 3g

Dilution Tested % Transmittance read for given dilution mL of DI-H2O used for grinding = 30 mL

No dilution %T = 9%,

mL of diastase added = 3 mL

1/5dilution %T = 50%,

Volume of filtrate = 33 mL

1/20dilution %T = 91%.

1/20 dilution which means , 1g / 2000 mL = 0.5%

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