Scientific Methodology : 5 Second Rule Experiment Lab Report Make sure each of t

Question

Scientific Methodology :

5 Second Rule Experiment Lab Report

Make sure each of the bold parts below are used as section headings in your lab report. The writing should be in a 12 font double-spaced and should not be longer than two to three pages (not including the tables).

Name: [Insert your Name Here]

Section: [Insert your Section Number Here]

Signature   ___________________________________    

Title:

(3 pts for name and creative title)

Introduction (7 pts)

In one large paragraph include the following:

Use references and provide three pieces of background information on the general characteristics of bacteria.

Then clearly state the hypothesis and prediction of your 5 sec drop experiment.

Use one or two sentences to connect your background information with the importance of your hypothesis and your prediction for the experiment.

Materials and Methods (5 pts)

Include Table 6

In a few sentences describe exactly how sampling was done for Part III and the conditions of incubation

Results (10 pts)

Results of Part II: Include the total number of colonies, and total number of different types of colonies that grew on each of the agar plates if possible.

Include Table 7, Hemolysis results table, and/or any other tables

Include drawings or photos

Describe what the bacterial growth looked like on each of the plates in general terms in a paragraph and make sure to refer back to your drawings or photos.

         

Discussion (12 pts)

Summarize the purpose of the 5 second rule experiment based on background information that you researched and read in two sentences.

Describe how the results supported or did not support the hypothesis and your prediction. Refer to data from the Results to do this. You may also need to explain why results were inconclusive. Don’t forget to compare the experimental plates to the controls (1 or 2 paragraphs).

Describe any problems with observing and quantifying the bacteria colony growth on the different plates in a sentence or two.

How do you think the properties of the food you tested affected this experiment? What other factors affected the results of this experiment? If you could repeat your experiment, what would you change or improve? (three sentences total)

Different species of bacteria have growth requirements that are specific to their needs. The needs vary greatly because bacteria grow everywhere from the hot springs of Yellowstone to even snow. Based on this perspective, do you think the same types of bacteria grew on the nutrient agar as the blood agar, why or why not? Do you have any proof of this from your experiment? Explain in a few sentences.

In a couple of sentences, write a conclusion about the 5 second rule in terms of what you learned from this experiment.

References (Minimum of 3 and don’t forget to cite them in report text) (3 pts)

NOTE>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

use this table and these agar type to do this assingment

gummy bear Part II: Results and Conclusions Record data in the table below. Take pictures of your plates using your phone (be careful!). Make a conclusion about your results. Did the able that you tested alter the number, diversity, type of bacterial colonies? Any additional of different Total of Colonies colony types based results Agar Type Plates on morphology hemolysis Nutrient 1: first half translu on Nutrient 1: second half 2: first half Tetracycline A 2: second half Tetracycline Blood 3: first half Circ, lar Moderate ref trans uarto Blood 3: second half Nutrient 5 Control Tetracycline Blood 6: ITA) 35

Explanation / Answer

TITLE-

Food Contamination:
Testing The 5 Second Rule

INTRODUCTION -The five-second rule, is a western cultural food hygiene concept, that states that there is a defined window where it is permissible to pick up food (or sometimes cutlery) after it has been dropped and thus exposed to contamination. Some may believe this assertion, whereas most people employ the rule as an amusing social fiction that allows them to eat a dropped piece of food, despite the potential reservations of their peers. HYPOTHESIS-: Foods that come in contact with dirty surfaces will have contaminates (Bacteria,
Fungus or Mold) on them.

MATERIALS REQUIRED-

A food source (Try moist food (luncheon meat) vs. dry food (dinner role).
Petri Dishes
LB Agar
Microwave (To heat the agar)
Q-Tips
Clean Water
Incubater (37 degrees Celsius)
Timer, stop watch or watch with second hand
500mL Flask
Scale and weigh boats
A known dirty surface (floor).

PROCEDURE-
1.Pour 250mL of water into the 500mL flask. Add ~ 10g of agar to the flask and heat for
1.5 min or until agar solubilizes with water. Pour liquid into petri dish (BE CAREFUL,
LIQUID IS VERY HOT). Allow to cool (~15-20 min)
2. Place food on contaminated surface for 2 sec. and 6 sec. Take the Q-tips and moisten it
with water run it across the surface of the food, particularly where the food touched the
dirty surface. Then run the Q-tip in a zig-zag fashion across the agar plate.   
Place plates into the incubater overnight at 37 degrees Celsius.
3. Compare the plates at various time points and count the colonies or estimate a percentage
of contamination for each plate. This is your data.

RESULT-

The reasoning for 2 and 6 seconds is because we are actually testing the time point of 5 seconds.
Two seconds represents a time point that is less than 5 seconds and if there is no contamination
at this time point then the 5 second rule stands. If there is contamination at 2 second, the myth is
busted.   
The sample taken at 6 seconds represents a time control. If 2 seconds is positive for
contamination then the 6 seconds should be also. This further busts the myth.   
If 2 seconds is negative but 6 seconds is positive then the 5 second myth stands and a time
greater than 5 is required for food to be contaminated.   
If both time periods are negative, then a greater time period is need to potentially have
contamination.

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