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You have isolated a temperature-sensitive mutant that, at the restrictive temper

ID: 91024 • Letter: Y

Question

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of the proteins forming the various transporters (TOM complex, SAM complex, TIM22 complex, TIM23 complex and OXA complex) and the porin proteins as candidates for the mutation. In other words, all the transporter proteins are wild type. What is your hypothesis for a protein that may be responsible for this mutant phenotype.

Explanation / Answer

The hypothesis for a protein that may be responsible for this mutant phenotype could be:

There occurs aminoacid substitutions in proteins at restrictive temperature that results in temperature sensitive mutant that has no ability to translocate proteins into mitochondria.

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