You have isolated a temperature-sensitive mutant that, at the restrictive temper

Question

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of the proteins forming the various transporters (TOM complex, SAM complex, TIM22 complex, TIM23 complex and OXA complex) and the porin proteins as candidates for the mutation. In other words, all the transporter proteins are wild type. What is your hypothesis for a protein that may be responsible for this mutant phenotype.

Explanation / Answer

Mitochondria plays major role in ATP production and has its own genome . Various proteins are encoded in nucleus and then need to be transported to mitochondria. The transporter proteins( TOM complex, TIM22 complex etc) performs that task.

So if this proteins transporters are wild type still translocation is not happening there is mutation in the Molecular chaperones(Heat shock protein 70 and Heat shock protein 90) definitely.

Hsp70 and Hsp 90 make proteins import competent and there by translocated by transporters protein into mitochondria.

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