The growth of the HPV was monitored as follows: 10 9 cells and 10 6 viruses in o
ID: 910416 • Letter: T
Question
The growth of the HPV was monitored as follows: 109 cells and 106 viruses in one milliliter were incubated under appropriate conditions. Following a first infectious cycle, a direct count and a plaque assay were performed. In the case of the direct count, the mixture was diluted by a factor of 1000X after which beads were added to obtain a final concentration of 1 X 104 beads/mL. A microscopic field of vision revealed 20 beads and 150 virions. In the case of the plaque assay, 67 plaques were observed on a plate on which 0.1 mL of a 10-5 dilution was assayed.
How many viruses would you expect to observe in the field of vision of the above described microscope for a 10-7 dilution of the supernatant after the last infectious cycle?
Explanation / Answer
Given: 67 plaques were observed on a plate on which 0.1 mL of a 10-5 dilution was assayed
Then if 10-7 dilution was done, the number will be = 0.67 plaques
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