What is the size of the W1 DNA insert? How many HinDIII sites are present in the

Question

What is the size of the W1 DNA insert?

How many HinDIII sites are present in the recombinant plasmid?

How many HinDIII sites are present in the W1 gene?

C) Chromosomal DNA containing the W1+ gene is cut with EcoR 1 and cloned into a 9kb plasmid (pUCSC) at its unique EcoRI site. Prof Robo decides to determine the restriction map of the recombinant plasmid. The circular plasmid that he generated is digested with either EcoRI or HinDIII. In addition, a reaction is performed in which EcoR1 and HinDIII are used together and the DNA fragments are resolved on an agarose gel shown below. Diagram a restriction map of this plasmid 2 155 155 13 13 11

Explanation / Answer

The W1 DNA is 13 kb in size.

There are two hind sites

There is one hind site in W1 gene

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