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There are seven chromosomes in the garden pea and seven independently assorting

ID: 91158 • Letter: T

Question

There are seven chromosomes in the garden pea and seven independently assorting RFLPs are identified in this organism. These results fit the hypothesis that each RFLP maps to a different chromosome. True/False cDNA libraries possess promoter sequences True/False PCR is a method that allows you to amplify a specific DNA sequence True/False Heterochromatin sequences would most likely be present in genomic libraries True/False The probability of isolating a gene from a cDNA library is not dependent on the cell type from which the library was originally constructed True/False

Explanation / Answer

(1) TRUE

Yes, it is true that each RFLP maps to a different chromosome, here the genes are coding for seven different characters, in this way all these genes will assort on different chromosomes.

(2) FALSE

cDNA will have all the information regarding the total clone. cDNA is the complementary DNA derived from mRNA, so cDNA will never carry information regarding introns, promoters, enhancers.

(3)TRUE

PCR is a popular technique mainly used to make copies of a specific DNA and it is one of most trusted technique for this purpose.

(4) TRUE

Yes, you can find the heterochromatin in genomic libraries and these regions will have tightly packed DNA sequences.

(5) FALSE

Yes, gene isolation from a cDNA library will depend on the cell type from which library was originally constructed as the cDNA will be synthesized from mRNA which are expressed in that cell types.

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