Determination of protein concentrations Determine the exact concentration of pro

Question

Determination of protein concentrations
Determine the exact concentration of proteins using Bradford assay (0.04 mL of standard/sample + 1.00 mL of Bradford reagent). Use BSA as a standard. You must build a new standard curve- Bradford is notoriously variable from day to day. Dilute the original samples with water if readings are not in the linear portion of the standard curve. Most often crude and FT give reasonable results hen diluted 1:100, and Wash1 and LDH1 fractions work at 1:20 dilution (change the dilution it depending on your results).

based on the privious paragraph answer the fallowing give extensive detailed
Why can’t BCA method be used for protein detection in this case?

How about Warburg’s method?

Explanation / Answer

1) We know that BCA method works on reduction of cupric ions in alkaline medium.

The difficulty in using BCA methods as compared to proposed method is it is susceptible to interference by some chemicals present in protein samples, including reducing agents and buffers with high concentration and also LDH.

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