The gene ust requires the transcriptional regulator AP-1 in order to be transcri

Question

The gene ust requires the transcriptional regulator AP-1 in order to be transcribed. AP-1 binds to an enhancer with the following consensus sequence --CGTTAGCTANGT--. Based on sequence data, it is also thought that the transcriptional regulator MEF2 acts as a competitive transcriptional repressor of AP-1 on this enhancer site. Using the data from the Electromobility Shift Assay (EMSA) below, answer the following questions: A. Does AP-1 bind to this consensus sequence? How do you know? B. Is MEF2 a competitive transcriptional repressor for AP-1? How do you know? C. Do the mutations made to the consensus sequence prevent all AP-1 binding? How do you know?

Explanation / Answer

A. AP1 binds to the consensus sequence, because there is a shift in the band when the mixture of AP1 and the consensus sequence is ran on EMSA

B. MEF2 is not a competitive inhibitor, because it does not show any shift in the binding of the substrate to the AP1 protein in its presence (Lane 3)

C. It does prevent the AP1 binding because there is a decreased binding of the protein to the substrate in the presence of mutation.

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