Isocitric acid is an intermediate product produced during the Krebs cycle, based

Question

Isocitric acid is an intermediate product produced during the Krebs cycle, based on the figure above answer the following questions: a. In the redox reaction depicted in figure 2, which compound is being reduced, and how did you determine this? b. In the redox reaction depicted in figure 2, which compound is being oxidized, and how did you determine this? 3. Assume you are working for a chemical company and are responsible for growing a yeast culture that produces ethanol. The yeasts are growing well on the maltose medium but are not producing alcohol. Why?

Explanation / Answer

Answer:

2. a. NAD+ is being reduced since it acts as hydrogen acceptor from C2 of isocitrate, during the catalysis of isocitrate to alpha-ketoglutarate

b. Isocitric acid is being oxidized since it donates hydrogen to the NAD+.

3. The most likely explanation is the presence of oxygen in the medium.

Yeast are facultative anaerobes. If you have O2 in the medium, the yeast can use it to run cellular respiration. If they do that, they won't undergo fermentation and would not produce alcohol.

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