In lab, the following stock solutions will be available: 0.1 M sucrose 0.2 M ace

Question

In lab, the following stock solutions will be available: 0.1 M sucrose 0.2 M acetic acid 0.2 M anhydrous sodium carbonate 0.2 M sodium acetate 0.2 M sodium bicarbonate Use the equation C_1V_1 = C_2V_2 to calculate V_1 of the components for each of the solutions below. Remember that in each case, C_1 is one of the stock solutions above, C_2 is the final concentration of each component and V2 is the final volume of the solution. Making unbuffered sucrose solution Calculate the amount of 0.1 M sucrose solution and the amount of dH20 needed to prepare 50 mL of unbuffered 20 mM sucrose. Write your answers in the spaces below and copy to your lab rewrite. Amount of 0.1 M sucrose solution needed _______ Amount of dH_2O needed to bring to final volume _______ Making Acetate buffer. Calculate the amount of each stock solution and the amount of dH_2O needed to prepare 50 mL of an acetate buffer that contains 20 mM sucrose, 40mM acetic acid, and 60 mM sodium acetate. Write your answers in the spaces below. Amount of 0.1 M sucrose solution needed _______ Amount of 0.2 M acetic acid solution needed _______ Amount of 0.2 M sodium acetate solution needed_______ Amount of dH_2O needed to bring to final volume _______

Explanation / Answer

A) Using the formula,

c1v1 =c2v2

Amount of 0.1 M sucrose needed is

(0.1) v1 = (20 x 10 -3) (50)

v1 = (0.02 x 50) / 0.1

v1 = 10

Therefore, the amount of 0.1 M sucrose needed to make 50 ml of 20 mM solution = 10 ml

The amount of dH20 needed to bring to the final volume = (50 - 10) ml = 40 ml

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