Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and the CRISP

Question

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and the CRISPR- associated protein (Cas) were originally identified in the Escherichia coli (E. coli enome, where they function as part of an RNA-based adaptive immune system to target and destroy genetic parasites the DNA The CRISPR/Cas9 in is simple compared to prior techniques for genome modification. In CRISPR/Cas9 targeted genome editing, you synthesize an RNA (gRNA) that can base pair with the DNA sequence targeting the host gene interest. When the gRNA is present i the cells, the gRNA can base pair with the DNA and the resulting RNADNA hybrid can be recognized by Cas9 when there is also a nearby protospacer adjacent motif (PAM) on the target. The canonical PAM is the sequence 5'-NGG-3' where "N" is any nucleotide followed by two guanine nucleotides. The activated and bound Cas9 endonuclease then causes double stranded breaks (DSBs) in the DNA in the area targeted by the gRNA base pairing. A double strand break triggers either of two mechanisms for repair: one is non-homologous end-joining (NHEJ). If homologous DNA is present in the cell (you can introduce DNA into the cell), then homologous recombination repair (HR), another mechanism for repair, can be activated Which of the following is FALSE regarding targeted genome editing with CRISPR/Cas9? CA CRISPR/Cas9 system components can be delivered to germ line cells to create heritable mutations B cRISPR genome editing can also be used to generate precise mutations in somatic tissues of adult animals. c The cRISPR/Cas9 system relies on proteins containing DNA-binding domains that recognize specific DNA sequences greater than 5 nucleotides in length D introduce a specific change within the for example a point mutation, that will a cause a specific amino acid substitution in the protein it is necessary to supply donor template that can be used for HR after Cas9 creates a DSB E CRISPR can be used to generate chromosomal rearrangements seen in human cancers.

Explanation / Answer

Option D is correct. CRISPR/Cas9 system can be used for both cell lines that is somatic as well as germ cell lines. It is used for chromosomal rearrangment in human cancer. It relies on protein containing DNA binding domain. Hence option D is correct.

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