energy per Figure Q3-45 -46 Chemical reactions are reversible; they can proceed

Question

energy per Figure Q3-45 -46 Chemical reactions are reversible; they can proceed in both the forward and directions. If the 4Go for reaction Y- x is favorable, how can you explain the fact that not all of the Y molecules will be converted to X molecules? 47 Enzymes A and B catalyze different reactions, but same reactant molecule as a use the substrate. The graph in Figure Q3-47 presents the reaction rates observed when enzyme A and enzyme Bare mixed together in a single test tube containing molecule X. are the Vmax and the apparent Km values for each enzyme under these conditions? How might these values change for enzyme B ifit were analyzed in the absence of enzyme A? Explain your answer. me Enzyme. 10 12.5 Concentration of XMMO Figure Q3-47

Explanation / Answer

In the double reciprocal plot as shown in the picture, the y intercept represents the reciprocal of Vmax and x intercept represents the negative reciprocal of Km.

y intercept = 1/Vmax

x intercept = -1/Km

For enzyme A, the y intercept is 0.16 and x intercept is -1 (which can be obtained by extending the line of enzyme A)

Therefore Vmax = 1/ y intercept

= 1/0.16

= 6

km = -1 / x intercept

= -1/-1

= 1

For enzyme B:

Vmax = 1/y intercept

= 1/0.1

= 10

Km = -1/x intercept

= -1/-0.33

= 3

Related Questions

Navigate

• Browse (All)
• Browse E
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.