Researchers are studying the use of RNA silencing as a medical application for g
ID: 91676 • Letter: R
Question
Researchers are studying the use of RNA silencing as a medical application for genetic diseases. Which of the following best describes how adding siRNA to cells would affect gene expression? a. It could activate a protein that fails to activate under normal conditions. b. It could replace a defective protein with a healthy one. c. It could increase the production of a protein that was under expressed. d. It could stop the production of a defective protein. Which of the following would lead to reduced gene expression for given gene? a. A primase inhibitor b. A proteasome inhibitor c. A histone deacetylase inhibitor d. Increased mRNA stability e. A histone acetyltransferase inhibitorExplanation / Answer
24. ANS: D.It could stop the production of a defective protein.
Explanation: siRNA functions by causing mRNA to be broken down after transcription (result is no translation). siRNA is used to decrease the gene expression in molecular biology experiments. It is inserted to nucleus with PEI or other reagents (Lipofectamine) and leads to degradation of RNA, thus decrease in production of the specific RNA. By targeting the mRNA, siRNAs will essentially decrease the translation of your gene of interest.
25. ANS: A. A primase inhibitor.
Explanation: Gene expression of human DNA polymerase alpha-DNA primase, a principal chromosomal replication enzyme complex, is up-regulated during the entrance of a cell from quiescence into the mitotic cell cycle. So the primase inhibitor can lead to reduce gene expression.
proteasomes are located in the nucleus and the cytoplasm. The main function of theproteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.
The histone deacetylase inhibitors are a new class of cytostatic agents that inhibit the proliferation of tumor cells in culture and in vivo by inducing cell cycle arrest, differentiation and/or apoptosis.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.