Use the following information to determine the sequence of an decapeptide. Prote

Question

Use the following information to determine the sequence of an decapeptide. Proteases and chemicals which break specific bonds in peptide sequences: Chymotrypsin, cuts on the C-terminal side of tyrosine, phenylalanine, and tryptophan. Trypsin, cuts on the C-terminal of lysine and arginine. Thermolysin, cuts on the N-terminal side of leucine, phenylalanine, isoleucine, and valine. Pepsin, cuts on the C-terminal of phenalaline, leucine, and glutamic acid. Cyanogen bromide, cuts on the C-terminal side of methionine. Information about the amino acids in this sequence: 1. The amino acid at the N-terminus is negatively charged with a 3 carbon side chain 2. The 2nd amino acid is the only non-chiral amino acid 3. One amino acid is the only polar neutral amino acid with sulfur in its R group 4. There are 2 positively changed amino acids and 2 negatively charged amino acids 5. A negatively charged amino acid with a two carbon side chain lies adjacent to Cystine 6. Histidine lays in-between Tyrosine and Methionine 7. Lysine lies adjacent to the only polar neutral amino acid with a benzene ring 8. The amino acid at the C-terminus is a polar neutral amino acid with a 1 carbon side chain and an OH group 9. Methionine is located at position # 7, and is adjacent to a negatively charged amino acid with a two carbon side chain. 10. One amino acid has the molecular formula of C_3H_7NO_2 and lies adjacent to glycine 11. A polar neutral amino acid is located next to the C-termis Experimental Results: A. Digestion with chymotrypsin resulted in 2 pentapeptides with neutral charges. B. Digestion with trypsin resulted in a tetrapeptide with a neutral charge and a neutral hexapeptide. C. Digestion with cyanogen Bromide resulted in a heptapeptide with a positive charge and a tripeptide with a negative charge. D. Digestion with thermolysin resulted in a decapeptide. E. Digestion with pepsin resulted in a negatively changed peptide and a positively charged nonpeptide.

Explanation / Answer

Negatively charged amino acid with 3 carbon side chain is glutamic acid. The only non chiral amino acid is glycine. Negatively charged amino acid with 2 carbon side chain is aspartic acid. Polar neutral amino acid with a benzene ring is tyrosine. Polar neutral amino acid with one carbon side chaiand one OH group is serine. Amino acid with molecular formula C3H7NO2 is alanine.

Considering these, the structure of decapeptide is as follows from N terminus to C terminus.

N terminus -Glutamic acid-glycine-alanine-lysine-tyrosine-histidine-methionine-aspartic acid-cysteine-serine- C terminus

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