1. Why will your fingers become dis colored if ninhydrin spray gets on your skin
ID: 917868 • Letter: 1
Question
1. Why will your fingers become dis colored if ninhydrin spray gets on your skin? 3. There are polarity and molecular weight differences between aspartic acid and phenylalanine: Aspartic acid has a polar, acidic side chain and a smaller molecular weight; phenylalanine has a nonpolar side chain and a larger molecular weight. Based on the Re values you obtained for these two amino acids in the solvent employed, which amino acid migrated faster and which of these two properties influenced the rate of migration?
Explanation / Answer
The Rf value of Aspartic acid is less than that of Phenylalanine.
So Phenylalanine migrates faster than Aspartic acid
Reason:
Absorption chromatography depends on physical forces such as dipole attraction to hold the molecules onto the surface of the solid packing.
Aspartic acid with the polar side chain is strongly attached / bonded to the polar adsorbent ( the stationery phase). Hence it moves slowly. Thus polarity of the molecule influences the rate of migration.
1)
The Ninhydrin Test is a test for amino acids and proteins with a free -NH2 group. When such an -NH2 group reacts with ninhydrin, a purple-blue complex is formed.
Skin contains amino acids. So ninhydrin stains/discolorize the skin blue
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