(a) What is the name of the experiment performed by reading output at detector A
ID: 923641 • Letter: #
Question
(a) What is the name of the experiment performed by reading output at detector A?
Circle all that apply: Absorption Emission Fluorescence Scatter Transmittance
(b) What types of spectroscopy are done by reading output at detector B?
Circle all that apply: Absorption Emission Fluorescence Scatter Transmittance
(c) What does detector B read if the analyte transmits or absorbs, but does not emit, scatter or fluoresce?
Circle one: high signal intensity low signal intensity zero signal intensity
(d) What does detector A read if the analyte is very slightly absorbing and non-fluorescent?
Circle one: high signal intensity low signal intensity zero signal intensity
(e) What does detector B read if the analyte is strongly fluorescing?
Circle one: high signal intensity low signal intensity zero signal intensity
Explanation / Answer
In absorption, atoms absorb part of the light from the source and the remainder of the light reaches the detector.
In emission, an excited atom can fall to a lower state and emit radiation.
Fluorescence is a emission of a photon during a transition between states with different spin quantum numbers.
(a) Since transmitted light is the reading out put at detector A, this experiment is absorption.
(b) Since emitted light is the reading out put at detector B, this experiment is either emission or fluorescence.
(c) Zero signal
(d) High signal intensity
(e) High signal intensity
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