Problem Title: Peptide Sequencing (Intro to Biochemichemistry) #1: Hydrolysis of
ID: 926924 • Letter: P
Question
Problem Title: Peptide Sequencing (Intro to Biochemichemistry)
#1:
Hydrolysis of dodecapeptide P with the enzyme trypsin affords the following fragments: Pro-Thr-Phe-Phe-Tyr-Ala-Arg, Asp-Asn-Arg, His-Arg.
Hydrolysis with chymotrypsin yields: Phe, Tyr, His-Arg-Pro-Thr-Phe, Ala-Arg-Asp-Asn-Arg.
What is the amino acid sequence of P? ___
________________
#2:
Hydrolysis of nonapeptide P with the enzyme trypsin affords the following fragments: Glu-Ser-Arg, Glu-Tyr-Ser-Phe-Ala-Arg.
Hydrolysis with chymotrypsin yields: Glu-Tyr, Ser-Phe, Ala-Arg-Glu-Ser-Arg.
What is the amino acid sequence of P? ___
Explanation / Answer
#1. Peptide P has is a 12mer (Dodeca) peptide.
Tyrpsin cleaves the peptide chain at Lysine and Arginine residues from C-terminal. Thus the residues obtained by trypsin hydrolysis are as follows:
Pro-Thr-Phe-Phe-Tyr-Ala-Arg, Asp-Asn-Arg, His-Arg
Chymotrypsin cleaves the peptide chain at aromatic residues viz. Tyrosine, Phenylalanine and Tryptophan from C-terminal. Thus the residues obtained by chymotrypsin hydrolysis are as follows:
Phe, Tyr, His-Arg-Pro-Thr-Phe, Ala-Arg-Asp-Asn-Arg
Hence the most probable amino acid sequence for peptide P will be:
His-Arg-Pro-Thr-Phe-Phe-Tyr-Ala-Arg-Asp-Asn-Arg.
#2. Peptide P has is a 9mer (Nona) peptide.
Tyrpsin cleaves the peptide chain at Lysine and Arginine residues from C-terminal. Thus the residues obtained by trypsin hydrolysis are as follows:
Glu-Ser-Arg, Glu-Tyr-Ser-Phe-Ala-Arg
Chymotrypsin cleaves the peptide chain at aromatic residues viz. Tyrosine, Phenylalanine and Tryptophan from C-terminal. Thus the residues obtained by chymotrypsin hydrolysis are as follows:
Glu-Tyr, Ser-Phe, Ala-Arg-Glu-Ser-Arg
Hence the most probable amino acid sequence for peptide P will be:
Glu-Tyr-Ser-Phe-Ala-Arg-Glu-Ser-Arg
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