Match the following biological techniques or terms with the best description of

Question

Match the following biological techniques or terms with the best description of their purpose or definition. Separates nucleic acid fragments or proteins based on size. Selectively replicates regions of DNA, using a very small amount of the original template. Can be used to splice two different strands of DNA together or to analyze RFLPs. Introduces selective mutations into a region of DNA. DNA resulting from the splicing of DNA from two or more different organisms. Determines nucleotide sequence for a region of DNA. a. PCR b. Site-directed mutagenesis c. Gel electrophoresis d. Recombinant DNA e. Sanger method f. Restriction digest

Explanation / Answer

1) Seperates nucleic acid fragments or proteins based on

size - c) Gel electrophoresis

2) Selectively replicates regions of DNA, using a very small amount of the original template - a) PCR

3) Can be used to splice two different strands of DNA together or to analyze RFLPs - f) Restriction digest

4) Introduces selective mutations into a region of

DNA - b) Site directed muta-genesis

5) DNA resulting from the splicing of DNA from two or more different organisms - d) Recombinant DNA

6) Determines nucleotide sequence for a region of

DNA - e) Sanger method

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