The general elution problem in chromatographic experiments is to (i) adequately

Question

The general elution problem in chromatographic experiments is to (i) adequately separate closely eluting species from each other, and (ii) to elute all species in the sample in a timely manner. How could you improve the separation illustrated below for each case.

(Don't only give a "key word". Briefly describe what you would do and why it might work. There may be more than one experimental change that would improve the separation.

b) a liquid chromatographic experiment?

c) a thin layer chromatographic experiment?

Explanation / Answer

a) a gas chromatography

Separation and elution time can be controlled by changing the flow rate of the mobile gas phase. A proper gradient run designed for chromatographic separation with temperature difference and rate of temperature change would yield a good separation of componenets of mixture.

b) a liquid chromatographic

Separation and elution of components by liquid chromatography can be controlled by choosing the right stationary phase and solvent or solvent(s) for the mobile phase. A solvent mixture which would be non-polar will elute non-polar components first and polar would help elute polar components first.

c) a thin layer chromatogaphy

Separation and elution in this case is very similar to the liquid phase chromatography. the type of stionary phase and the combination of solvents for the mobile phase would yield a good separation depending on the type of compounds present in the mixture.

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