Ethidium bromide causes mutation by: a) Inserts between paired bases b) Competes
ID: 93031 • Letter: E
Question
Ethidium bromide causes mutation by: a) Inserts between paired bases b) Competes with natural bases c) Removes amino groups from bases d) Cause strand break e) Form thymine diners Base analog causes mutation by: a) Inserts between paired bases b) Competes with natural bases c) Removes amino groups from bases d) Cause strand break e) From thymine dimmers Ultraviolet light causes mutation by: a) Inserts between paired bases b) Competes with nature bases c) Removes amino groups from bases d) Cause strand break e) From thymine dimmers Frame shift mutations causes: a) Alternation of reading frame of entire protein from the point of mutation b) Change of codon to a stop codon c) Change of a codon that will cause incorporation of same amino acid d) Addition of one or more base e) Deletion of one or more base Nonsense mutation causes: a) Alteration of reading frame of entire protein from the point of mutation b) Change of codon to a stop-codon c) Change of aExplanation / Answer
19)Answer -A: EtBr is a chelating agent, which means it binds to DNA by inserting itself into the 2 strands of DNA and deforms it.
20) Answer-B: The term analog refers to "similar", and base analogs are similar to natural bases and can be substituted in its place. Thus they compete with natural bases.
21) Answer-E: Thymine dimers are otherwise known as "thymine photo-dimers" caused by UV radiation. These rays enhance the covalent link formation among adjacent thymine bases and deform DNA.
22) Answer- A: A frameshift mutation is caused by addition or deletion of one or more bases, thereby shifting the reading frame of entire protein from the point of mutation.
23)Answer-B: Nonsense mutation means making a sense codon a non-sense codon ie., a stop codon.
24)Answer-D: A single nucleotide base may be altered by addition/deletion/substitution is known as the point mutation.
25)Answer-C: Transformation is otherwise commonly known as bacterial transformation. ie., introducing DNA into Bacteria.
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