chromatography question Student C calls fractions, and he shows you over and exp
ID: 932589 • Letter: C
Question
chromatography question Student C calls fractions, and he shows you over and explains that after running his column, he collected 5 fractions, and he shows you the TLC of the fractions: Identify which of the following scenarios that could have led to him obtaining this TLC Explain why each scenario may or may not lead to student C's TLC. Student C loaded his sample onto the column diluted with too much solvent. Would this scenario lead to the above TLC? Yes or No. Why? Student C used too nonpolar an eluent system. Would this scenario lead to the above TLC? Yes or No. Why? Student C used too polar an eluent system. Would this scenario lead to the above TLC? Yes or No. Why? Student C used too little adsorbent. Would this scenario lead to the above TLC? Yes or No. Why? Student added eluent prior to material adsorbed into silica. Would this scenario lead to the above TLC? Yes or No. Why? Student C used a column that had too large a diameter. Would this scenario lead to the above TLC? Yes or No. Why?Explanation / Answer
A. No. Adding too much solvent will not ellute two components together as there is a considerable difference in the Rf value of the components.
B. No. If a too non polar solvent is used the component with higher Rf value will be elluted first. After a large volume of solvent is applied only then the component with lower Rf value will be elluted.
C. Yes. If a too polar solvent system is employed the two components will come out together and they can not be seperated.
D. Yes. If too litle adsorbent is used the solvent will have to pass a small distance before ellution. A small elluton time implies poor seperation.
E. Yes. The material should be adsorbed properly into silica to achieve an egfficient separation.
F. No. The diameter of the column has no role in the seperation of the omponents.
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