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Which one of the following would you inoculate if you wanted to keep the culture

ID: 93414 • Letter: W

Question

Which one of the following would you inoculate if you wanted to keep the culture for several weeks in refrigeration?

Select one:

a. Liquid broth

b. Vortex

c. Glass tube

d. Agar slant

What is a major difference between the quadrant streak and zigzag inoculation method?

Select one:

a. Using a sterile instrument for streaking after the initial streak

b. Identifying a mixed culture

c. Amount of space of plate the experiment uses

d. Type of agar that can be used

e. Possibility of cutting the agar

You streak an unknown sample and after incubation you see some red colonies and some yellow. What can you interpret from this experiment?

Select one:

a. Likely started with a pure culture

b. A spread plate would work better

c. You have identified Enterobacter aerogenes

d. You have identified Serratia marcescens

e. Likely started with a mixed culture

On a typical plate (100 cm^2), you spread 600 cells. What would likely be your observation after a couple days of incubation?

Select one:

a. Achieved isolated colonies throughout

b. Cells did not grow

c. Only a few cells grew

d. Too many cells for isolated colonies

e. Cells will start anti-fermentation

If you want to isolate a colony from a culture with a high cell density, which of the following would be preferred?

Select one:

a. Spread plate method

b. Zigzag pattern method

c. Quadrant streak method

d. Triple streak method

e. Liquid inoculation

Why would you hold a glass tube with liquid broth at an angle during the process of inoculation?

Select one:

a. To grip the tube better

b. To minimize spilling

c. To minimize contaimination, such as air-borne microbes

d. The protect your eyes

After inoculating the loop through the flame, why does wire need to cool?

Select one:

a. Can liquify the wire

b. Can liquify the entire agar in tube

c. Can kill microbes and cause aerosols of microbes

d. Can cause overgrowth of bacteria

e. Can start a fire

Explanation / Answer

1) agar slant.

2) Using a sterile instrument for streaking after the initial streak

3) Likely started with a mixed culture

4) Too many cells for isolated colonies

5) Quadrant streak method

6) To minimize contamination, such as air-borne microbes

7) Can kill microbes and cause aerosols of microbes

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