HYDROLYSIS OF STARCH BY AMYLASE Pre-Lab assignment SUBMIT THIS ASSIGNMENT at the
ID: 93543 • Letter: H
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HYDROLYSIS OF STARCH BY AMYLASE Pre-Lab assignment SUBMIT THIS ASSIGNMENT at the beginning of lab on 06/22/2017 Your responses should be neatly written or typed Late submissions will not be accepted. This assignment is worth 8 points NAME: Define enzymes? What are they made of? 2. How do enzymes catalyze reactions in living cells? 3. Why is it important to have enzymes catalyze biological reactions? 4. What environmental factors affect enzyme activity? 5. What is the relationship between enzyme activity and its 3D structure https lob-montgomery college blackboard.com/bbcswebdavpi 519.1/courses/40460201740MEnzymex20Pre-Labs20su17 docx 6/2 12 14 PM Page 1 of 2Explanation / Answer
1) enzyme are the biological catalyst. they act upon the substrate and change them into product without being consumed or change.
2) they convert the substrate into the product and catalyse the reaction by lowering the activation energy.
3) they are very selective and specific. energetically favourable reaction. enhance the rate of reaction many folds.
4) temperature, pH, cofactor, inhibitors, the concentration of enzyme and substrate.
5) 3D structure means active site formation. if active site is not formed properly or enzyme denatured. so activity hampers or no activity at all.
6) the optimal temperature of an enzymatic reaction is the temperature at which conversion of substrate into product is maximum.
7) optimal pH means the pH at which enzyme is stable and maximum active.
8) 9) and 10) you have to answer by yourself.
11) during enzymatic reaction substrate concentration decreases, product concentration increases and enzyme concentration remain unchanged.
12) Maltose is produced by hydrolysis of starch.
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