Discovered a gene from cancer cells and found to be associated with development

Question

Discovered a gene from cancer cells and found to be associated with development of cancerous cells. The nucleotide sequence of the template strand was determined as written below study the sequence and answer the following question. 3'GACACGTACAAATTTGAGCCTGGACACCTTAAGAGCGGGCTCGGAACACTGGCCCCGATTGACAC 5' A. Write down the sense strand of the gene showing direction. B. Write down the nucleotide sequence of the mRNA. C. How many amino acids will be in polypeptide produced from this mRNA? Write down the amino acid sequence of the polypeptide. D. Write down the sequence of the nucleotides of the DNA fragments produced from the after EcoRI digestion. (EcoRI recognition site is GAATTC)

Explanation / Answer

Answer:

A. The sense strand has the same sequence as the mRNA and complementary to the template strand. So, the sequence of the sense strand is:

5' CTGTGCATGTTTAAACTCGGACCTGTGGAATTCTCGCCCGAGCCTTGTGACCGGGGCTAACTGTG 3'

B. The nucleotide sequence of the mRNA is:

5' CUGUGCAUGUUUAAACUCGGACCUGUGGAAUUCUCGCCCGAGCCUUGUGACCGGGGCUAACUGUG 3'

C. The mRNA would code for 17 amino acids in the polypeptide.

M F K L G P V E F S P E P C D R G Stop

D. EcoR1 cleaves at GAATTC and produces 5' AATT overhang. In the sense strand, EcoR1 cleaves at 28th nucleotide position. (Since nothing has been mentioned in the question, the sense strand has been taken into account)

Sequence of the nucleotides of the DNA fragments produced after EcoRI digestion are:

i. 5' CTGTGCATGTTTAAACTCGGACCTGTGG 3'

ii. 5' AATTCTCGCCCGAGCCTTGTGACCGGGGCTAACTGTG 3'

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