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Biochemical test a. catalase Motility test b. urease Hydrogen peroxide c. pure c

ID: 93638 • Letter: B

Question

Biochemical test a. catalase Motility test b. urease Hydrogen peroxide c. pure culture Urease d. semi-solid (7%) media Phenylalanine e. deaminase State if each of the following is True or False. Only a mixed culture will give a positive result. All biochemical used in the lab have a built in indicator. All bacteria is indole positive. All bacteria are capable of producing enzyme. Exo enzymes are produced by some bacteria More than one biochemical activity can be obtain in certain tube. A positive urea test indicate the present of the genus Proteus. Some bacteria can produce caseinase. All bacteria can produce lipase Indole can be produced from the amino acid tryptophan phase.

Explanation / Answer

Semi-solid media are used to determine the motility of bacteria. Growths in non-motile bacteria are confined to the stab-line. They have sharply defined margins and the surrounding medium is transparent. Motile bacteria typically give diffuse, hazy growths that spread throughout the medium rendering it opaque.

Phenylalanine deaminase test is also known as phenylpyruvic acid (PPA) test. It is used to test the ability of an organism to produce enzyme deaminase.

16) Biochemical test - catalase

17) Motility test - semisolid (7%) media

18) Hydrogen peroxide - pure culture

19) Urease - urease

20) Phenylalanine - deaminase