The transcription activator protein GAL4 has three functionally distinct regions

Question

The transcription activator protein GAL4 has three functionally distinct regions. Explain what happens to the following three yeast strains with respect to galactose gene regulation and the strain's ability to grow on galactose. a) Strain one has a mutation that inactivates the GAL80 binding region of GAL4 so that it cannot bind the GAL80 protein. b) Strain two has a mutation that inactivate the DNA binding region of the GAL4 protein. c) Strain three has mutations that delete the Gal 1 and Gal 3 genes.

Explanation / Answer

GAL80 IS A REPRESSOR...SO WHEN IT CAN'T BIND THERE IS MORE ACTIVITY OF GAL 4 IS NOTED....SO MORE PRODUCTION OF GALACTOSE

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