You are conducting research on a new Hfr strain in E. coli that you have develop

Question

You are conducting research on a new Hfr strain in E. coli that you have developed and obtain the following data. Use the data to give the order of the genes on the bacterial chromosome starting with E and going clockwise and indicate the minimum distances between them in minutes based on the chart. After publishing the data above in a research journal you are contacted by a lab in Canada. Working with the other lab you generate additional Hfr strains and allowed them to incorporate into the bacterial genome. Hoping the new strains will allow you to map additional genes the bacteria were allowed to start conjugation resulting in the following set of gene transfers for each strain. E S ^C A N middot R E R E P L I C A C A N middot R E P L I E R middot N A C ^S Use the results from your conjugation experiment to determine the order of genes on the circular bacteria chromosome Starting with E in Hfr 2 and going clockwise, include the insertion site for each Hfr strain and its polarity (direction).

Explanation / Answer

The order of genes on bacterial chromosome will be E, P, I, S, O, M, E, S, A with time of 9, 3.5, 4, 4, 5, 4.5, 4, 5, 4.5 respectively.

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