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Exercise 1: Paper Chromatography Data Table 1. Photographs of Chromatograms. Pho

ID: 944006 • Letter: E

Question

Exercise 1: Paper Chromatography

Data Table 1. Photographs of Chromatograms.

Photograph of Chromatogram #1

Photograph of Chromatogram #2

Data Table 2. Chromatogram Sheet 1 Data.

Sample

Solvent front (mm)

Spot 1

Spot 2

Spot 3

mm

Rf

mm

Rf

mm

Rf

Blue 1

Blue 2

Red 3

Red 40

Yellow 5

Yellow 6

KG

KS

Data Table 3. Chromatogram Sheet 2 Data.

Sample

Solvent front (mm)

Spot 1

Spot 2

Spot 3

mm

Rf

mm

Rf

mm

Rf

Store Red

Store Yellow

Store Green

Store Blue

Unknown

M&M: M -_

M&M: M -_

M&M: M -_

M&M: M -_

Data Table 4. FD&C Dyes in Samples.

Sample:

FD&C Dyes in Sample

Kool-Aid Grape ®

Kool-Aid Strawberry ®

Store Red

Store Yellow

Store Green

Store Blue

Unknown Sample

M&M®__________

M&M®__________

M&M®__________

M&M®__________

Questions

      What might happen you used a pen to mark the baseline on the chromatography paper?

      What would happen if you did not stop the chromatograph before the solvent reached the top of the paper?

      What would happen if the solvent level was above that of the dye spots on the paper at the start of the chromatography?

     Why is it important to mark the solvent level on the chromatography paper as soon as you remove it from the Petri dish?

Photograph of Chromatogram #1

Photograph of Chromatogram #2

Explanation / Answer

1.

A pen should not be used to draw the baseline on the chromatogram because the pen ink will dissolve and would be separated and can mix with the dyes and interfere with the results.Thus a pencil should be used instead.