You measure your enzyme’s activity at several different substrate concentrations

Question

You measure your enzyme’s activity at several different substrate concentrations (see data below), but the enzyme’s activity is unchanged by substrate concentration.

[S] (mM)

vo (µM/min)

1 x 101

295

1 x 102

298

1 x 103

301

1 x 104

302

a) Why is the activity of your enzyme not changing with increasing substrate concentrations, and how could you address this problem so that you could generate the typical hyperbolic v vs. [S] plot (Michaelis-Mentin plot)?

[S] (mM)

vo (µM/min)

1 x 101

295

1 x 102

298

1 x 103

301

1 x 104

302

Explanation / Answer

The enzyme become poisoned or all the active sites of enzymes are blocked by substrates.

We conclude this by drawing a graph which shows that at perticulat conc of sustrate the enzyme activite is increases wirth substrate. After some time the curve become parallel to enzyme activity axis.

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