You are trying to get a better understanding of why the Oct4 transcription facto

Question

You are trying to get a better understanding of why the Oct4 transcription factor is so important for regulation of stem cell identity. You decide to use a CHiP-seq assay and a yeast two hybrid assay to gain a ‘genome wide’ understanding of the function of this protein. Explain the different types of information you will get from these two assays and how you would set each one up in order to understand how this protein interacts with the rest of the genome (and/or proteome). You are trying to get a better understanding of why the Oct4 transcription factor is so important for regulation of stem cell identity. You decide to use a CHiP-seq assay and a yeast two hybrid assay to gain a ‘genome wide’ understanding of the function of this protein. Explain the different types of information you will get from these two assays and how you would set each one up in order to understand how this protein interacts with the rest of the genome (and/or proteome).

Explanation / Answer

Genome is approaching and are saturating sequence database. Signal transduction pathway might be considered as large protein structures through which the signal is transmitted.The newly formed complex is capable of transmitting multiple specific signals provoking a highly regulated cellular response.Alteration of protein protein interactions is known to contribute many diseases.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.