The sequence below represents the first 9 bases of a primary RNA transcript from

Question

The sequence below represents the first 9 bases of a primary RNA transcript from a bacterial gene. Which of the following represents the correct CODING strand for the corresponding DNA sequence? 5'-GACUAGCUU-3' A) 5'-GACUAGCUU-3' B) 5'-GACTAGCTT-3' C) 3'-GACTAGCTT-5' D) 5'-CTGATCGAA-3' E) 3-CTGATCGAA-5' Which of the following correctly pairs a biotechnology tool with its use? A) PCR: a technique used to identify specific RNA sequences B) Replica plating: a test to determine the mutagenic activity of chemicals C) Restriction digest: a technique used to make multiple copies of a segment of DNA D) Molecular cloning: use of variable restriction digest fragments for DNA identification E) None of the above Which of the following primers would you need to order if you wanted to amplify the entire DNA shown below? Pay attention to 5' and 3' orientation. 5' CAGTATGCACGGCGGCGGCGGATGCGTAAT 3' 3' GTCATACGTGCCGCCGCCGCCTACGCATTA 5' A) 5'-ATTACGCAT-3' and 5'-GTCATACGT-3' B) 5'-ATTACGCAT-3' and 5 -CAGTATGCA-3' C) 5'-ATGCGTAAT-3' and 5 -GTCATACGT- 3' D) 5 '-ACGTATGAC-3' and 5'-TACGCAITA-3' E) 5'-TGCATACTG-3' and 5'-TAATGCGTA-3' A bacterial cell transfers chromosomal genes to F- cells, but it does not cause them to become F^+. The bacterial cell is: A) Hfr. B) lysogenic. C) auxotrophic. D) lytic E) F

Explanation / Answer

The answer for qeustion 1:

in RNA, G is the complemetary base for C or vice versa and
A is the complementary base for U or vice versa.
Non template strand is called as coding strand. Which is almost similar to mRNA transcript except in T base.

So, the answer is B. 5'gactagctt3'

The answer for question 2:

PCR for invitro amplification of DNA molecules.
For identifcation of sepecific RNA sequences Nothern blotting will be employed.
so, the option A is WRONG.

Replica plating is meant for creaing duplicate plates as like mother culture.
To determine the mutagenic acvitivity of chemicals Ames test will be employed.
So, the option B also WRONG.

By using Restriction Digestion technique we can cut the large nucleic acid in to small fragements.
By using PCR we can make multiple copies of segement of DNA.
So, The option C also wrong.

Molecular cloning - we can prepare multiple copies of wanted DNA using this technique.
VNTR is the technique by using this technique we can identify the DNA by using variable restriction digestion.
So, the option D also wrong.

Hence the option E: NONE OF THE ABOVE is right answer.

ACCORDING TO CHEGG GUIDELINES WE HAVE TO ANSWER ONE QUESTION AT A TIME. SO POST THE REST AS SEPERATE QUESTIONS THEN I CAN HELP YOU.

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