o Verizon 8:09 PM 35% Isidore Thin-Layer Chromatography 6. Identify the stationa

Question

o Verizon 8:09 PM 35% Isidore Thin-Layer Chromatography 6. Identify the stationary and mobile phases of the TLC from this experiment. 7. Explain the resulting trend of Rf values for the various components of the spinach extract based on the principles of thin-layer chromatography. Address both the stationary and mobile phases 8. What are two common methods used to visualize colorless compounds on a TLC plate based on the reading? 9. TLC can be used in six applications of organic chemistry. Which two applications were used in this experiment? Justify your

Explanation / Answer

6. The stationary phase in the tlc here is thin layer of silica gel. Mobile phase is acetone-Hexane mixture.

7. Since the mobile phase acetone-hexane is non-polar phase, the non-polar components would preferentialy absorb in this phase and would move faster on the tlc plate. Carotenes are the most non-polar component would move fastest on the plate followed by chlorophyll-A which is more non-polar than chlorophyll-B. Chlorophyll-B would stay on the silica gel stationary phase for longer times (like-like interaction).

8. The two best ways to visualize colorless compounds on a TLC plate are, (a) Iodine stain and (b) UV-Vis light.

9. In the current experiment, TLC was employed to (a) qualitative separation of components of the mixture and (b) quantitative separation of components by removing silica of the spots and dissolving in proper solvent.

Related Questions

Navigate

• Browse (All)
• Browse O
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.