The TLC chromatogram shown below contains glycine (G), isoleucine (l), valine (V

Question

The TLC chromatogram shown below contains glycine (G), isoleucine (l), valine (V) and a sample for identification (S-1). Select the most accurate statement from the list of possibilities concerning this chromatogram. The sample S-1 contains glycine, isoleucine, and valine. The sample S-1 contains both glycine and isoleucine but not valine. The sample S-1 contains glycine but not isoleucine or valine. The sample S-1 contains both isoleucine and valine but not glycine. A student could not identify which suspect spots matched the evidence spots on the TLC plate. Which of the following are reasonable possible sources of error? Pen instead of pencil was used to mark the TLC plate. The developing solvent resulted in spots with RfS below 0.3 and above 0.7. The student measured solvent incorrectly when making the developing solvent. The spot of sample placed on the TLC plate was too large. The developing solvent covered the sample spots at the application line. The solvent front was 50% up the plate.

Explanation / Answer

Glycine and isolucine are the amino acids which can be detected but not valine

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