You think you may have hit upon a gold mine in the newly identified antibiotic.

Question

You think you may have hit upon a gold mine in the newly identified antibiotic. You want to secure a private investor to help fund a biotech company startup. But before you can convince a billionaire to put up the funds, you have to make some changes to your microorganism. A major challenge in bio-production is minimizing biomass and maximizing product - essentially you want the cell to move more carbon away from making cellular components and into making the excreted product of the antibiotic without killing the cell. The antibiotic is an energy costly product so you must setup ideal conditions for maximum growth/production. You deduce that the antibiotic is a nucleotide analog and uses the same biosynthetic pathway as guanosine monophosphate (GMP). How can you genetically manipulate the organism to increase production of the antibiotic and decrease cellular biomass and grow it at a maximum output? To grow the microorganism, what type of carbon and energy would you provide along with oxygen conditions? Include clues from the case study to explain your answer.

Explanation / Answer

7. Carbon is provided in the form of whether they may be proteins, fats, carbohydrates or lipids along with oxygen conditions.

Oxygen is used by aerobic bacteria during the process of cellular respiration as a final electron acceptor.

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