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Give three suggestions that your university could implement to make the lab a \"

ID: 958693 • Letter: G

Question

Give three suggestions that your university could implement to make the lab a "greener" place. 2. How can flavonoids (anthocyanindins) be used as natural acid-base indicators? 3. If a natural indicator is added to distilled water, what, if any change(s) would you expect to occur? 4. How would you classify most household cleaning products? Justify your answer. a. acidic b. basic c. neutral d. none of the above. 5. Sorghum bicolor indicator is added to a solution containing caffeine to be titrated. The caffeine solution was colorless but when the indicator was added, the solution turned a deep pink color. Estimate the pH of the caffeine solution. Is caffeine acidic or basic based on the observation of the color change?

Explanation / Answer

Anthocyaninwas extracted from rose flowers by 3 methods using methanolicsolutions of 0.1% HCl, citric acid and tartaric acid.

The anthocyaninwas used as natural indicator in acid –base titration.

The intrinsic pH of the extract was 2.88.

Rose anthocyaninextract showed wavelength maxima change from 517 nm (dark pink) to 592 nm (green) between the pH range 2 to 9.

The colorant present in the rose flower is mainly consisting of cyanidin, pelargoninor peonidinor to mixtures of these pigments.

In acidic aqueous media (pH 29), there are three forms of cyanidin: the flavyliumcationAH+, the carbinolB, and the quinonoidalbase A.

Equilibrium between the two neutral forms occurs exclusively by way of the flavyliumcation.

Hydration of the flavyliumcationinvolves the formation of a CO bond and a proton transfer.

The existence of very small amounts of a third neutral form, the chalconeC, a prototropictautomerof the carbinolB, has also been reported.

Rose anthocyanincolor scale on different pH show colors from pink to green.

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