Fill out Table A below in your lab notebook. Make sure you account for the extra

ID: 958916 • Letter: F

Question

Fill out Table A below in your lab notebook. Make sure you account for the extra volume beyond the 200 muL in triplicate (100 muL should be sufficient). Standard Curves and Spectrophotometry Stock solution of Bromophenol Blue is 28.95 muM Make the following standard curve: Use the M_1V_1=M_2V_2 equation. Or M_final *Vol_final=M_stock *Vol_stock M=concentration V=volume Make enough so you can load 200 muL in triplicate on your microplate plus accounting for at least 100 muL of extra volume. Measure the concentration of the three unknowns Load your blank, standard curve, and other solutions into your microplate in triplicate. Read the plate at a wavelength of 590 nm. Show the instructor an excel file with your data and quantification of the 3 unknowns.

Explanation / Answer

Formula: Mfinal*Volfinal = Mstock*Volstock

Mstock is the stock solution concentration (28.95 uM).

Amount of stock (Volstock) = (Mfinal*Volfinal)/ Mstock

Amount of water = Volfinal - Volstock

Volfinal is final solution volume = 100 uL.

Concentration (: Mfinal), uM

Amount of stock (Volstock), uL

Amount of water , uL

69.08

30.91

51.81

48.19

34.54

65.46

17.27

82.73

6.91

93.09

3.45

96.55

0.5

1.73

98.27