\"Aseptically place one or two drops of hydrogen peroxide onto the bacteria and

Question

"Aseptically place one or two drops of hydrogen peroxide onto the bacteria and immediately observe for the formation of bubbles." In this step, what is the rationale for using a sterile technique? Choose the most likely answer.

Select one:

a. Catalase-negative bacteria could give a false positive

b. Catalase-positive bacteria could give a false positive

c. Bacterial contamination will grow in the hydrogen peroxide

d. A stray bacterium will weaken a lot of peroxide

e. Catalase-negative bacteria could give a false negative

In which aspect of ATP production is the oxidase enzyme present?

Select one:

a. Electron transport chain

b. Kreb's Cycle

c. Lipolysis

d. Glycolysis

e. Glyconeogenesis

Catalase test: Which of the following is NOT true about the ETC?

Select one:

a. It is found in obligate anaerobes

b. Oxidative phosphorylation occurs there

c. ADP is converted to ATP there

d. Oxygen is converted to water there in aerobic respiration

e. It is found in obligate aerobes

Explanation / Answer

1) The enzyme catalase test decomposes hydrogen peroxide to oxygen and water. The presence of catalase enzyme can be detected by adding hydrogen peroxide to the bacterial medium. While doing the catalase test, it is important to aseptically place one or two drops of hydrogen peroxide onto the bacteria and immediately observe for the formation of bubbles because catalase negative bacteria may give false positive results.

2) The enzyme cytochrome c oxidase is present in electron transport chain which is involved in the production of ATP from ADP. Therefore, the answer for this question is option (a) electron transport chain.

3) Electron transport chain occurs in the presence of oxygen. Here, oxygen getsconverted to water in aerobic respiration. Oxidative phosphorylation of NADH and FADH2 also occurs producing ATP molecules. So, the answer for this question is option (a) it is found in obligate anaerobes.

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