. Performing a complementation test through cotransduction using mutant phage ge

Question

. Performing a complementation test through cotransduction using mutant phage genomes created by X-ray mutagenesis (mutants a-e), as well as ones created by chemical mutagenesis using EMS (mutants 1-5), you get the following results:

1          2    3    4 5

      a    — +    + — —

      b    — +    — — —

      c    +   + — — +

      d    +    — +    +    —

      e     — — +    + —

Explain this data.

Hint: X-rays create DNA breaks that lead to deletions, and chemical mutagens such as EMS result in point mutations.

Explanation / Answer

X-rays create DNA breaks that lead to deletions, and chemical mutagens such as EMS result in point mutations. Thus, mutants a-e are deletion mutants and 1-5 are point mutants. Analysis of the data can provide us with a comparative map of the mutants as follows

Related Questions

Navigate

• Browse (All)
• Browse #
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.