A peptide with nine amino acids was divided into three samples and each sample w

Question

A peptide with nine amino acids was divided into three samples and each sample was subjected to (i) cyanogen bromide, (ii) chymotrypsin. or (iii) trypsin treatment. The fragments obtained in each of the treatments were separated, hydrolyzed and subjected to amino acid composition analysis. What Ls a possible sequence for each fragment or the actual placement (# 1 - # 9) of each amino acid residue (if either can be deduced from information given at each point!. If no specific information about the sequence can obtained from the data, leave it blank. Explain the reasoning for your answer. Cyanogen bromide fragment 1: isolcucine. methionine, tyrosine, valine Cyanogen bromide fragment 2: alanine, cysteine, glycine, lysine, tryptophan Possible sequence: Explanation:

Explanation / Answer

Cyanogen bromide hydrolyzes peptide bonds at the C-terminus of methionine residues. So the second fragment must be attached to the methionine residue.

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