Whole genome or shotgun sequencing requires the creation of a cDNA library. the
ID: 96685 • Letter: W
Question
Whole genome or shotgun sequencing requires
the creation of a cDNA library.
the creation of overlapping DNA fragments whose sequences can be aligned into large contiguous fragments (contigs) using computer algorithms.
large contiguous fragments (contigs) cloned into yeast artificial chromosomes (YACs) in an ordered fashion.
very large amounts of DNA from the organism whose genome is being sequenced.
the use of a gene gun to propel DNA-coated particles at high velocities into cells.
a.the creation of a cDNA library.
b.the creation of overlapping DNA fragments whose sequences can be aligned into large contiguous fragments (contigs) using computer algorithms.
c.large contiguous fragments (contigs) cloned into yeast artificial chromosomes (YACs) in an ordered fashion.
d.very large amounts of DNA from the organism whose genome is being sequenced.
e.the use of a gene gun to propel DNA-coated particles at high velocities into cells.
Explanation / Answer
Shotgun sequencing is used for sequencing of long DNA strands. Here, the DNA strands are broken down into smaller fragments which can be sequenced individually. The sequenced fragments are then assembled together by the computer to find where the fragments overlap. Shotgun sequencing is a much faster process than the clone by clone sequencing. Therefore, the answer for this question is shotgun sequencing requires the creation of overlapping DNA fragments whose sequences can be aligned into large continuous fragments using computer algorithms (option b).
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