Raffinose is a trisaccharide commonly found in peace, beans, and other vegetable
ID: 967941 • Letter: R
Question
Raffinose is a trisaccharide commonly found in peace, beans, and other vegetables. Consider the structure of raffinose and answer the following questions.
A. What observations would you expect when raffinose is treated with the following reagents?
Benedict's______________
Barfoed's ______________
Seliwanoff's ___________
Iodine ______________
B. If a sample of raffinose werr completely hydrolyzed, what observations would you expect when the product mixture is treated with the following reagents?
Benedict's ____________
Barfoed's _____________
Seliwanoff's ___________
Iodine _________________
C. Which of the Regents from above provides evidence that the raffinose sample was successfully hydrolyzed? Explain your answer.
Explanation / Answer
A.
What observations would you expect when raffinose is treated with the following reagents?
Benedict's Raffinose is an example of reducing sugar it made of galactose, glucose, and fructose. Benedict's reagent reacts with reducing sugars, hence it gives brick red color with raffinose also .
Barfoed's gives negative result because it reacts with monosaccharide only.
Seliwanoff's gives negative result because this reagent reacts with ketoses carbohydrates.
Iodine gives negative result
B. If a sample of raffinose werr completely hydrolyzed, what observations would you expect when the product mixture is treated with the following reagents?
Benedict's Benedict's Raffinose is an example of reducing sugar it made of galactose, glucose, and fructose which are free on hydrolysis and all are reducing sugars Benedict's reagent reacts with reducing sugars, hence it gives brick red color with galactose, glucose, and fructose.
Barfoed's after hydrolysis we will get galactose, glucose, and fructose and it gives positive result because it reacts with monosaccharide only.
Seliwanoff's after hydrolysis we will get galactose, glucose, and fructose and fructose gives positive result with Seliwanoff's because fructose is a ketose.
Iodine negative result because is a test for the presence of starch.
C. Which of the Regents from above provides evidence that the raffinose sample was successfully hydrolyzed? Explain your answer.
Seliwanoff's because it reacts only ketose which we will get after successfully hydrolysis of raffinose.
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