Hint: Affinity chromatography uses biologically selective and reversible interac

Question

Hint: Affinity chromatography uses biologically selective and reversible interactions to retain and detect analytes of interest. The stationary phase is an immobilized molecule called the affinity ligand, which has a high affinity for the analyte of interest. Examples of these interactions include an enzyme and its substrate, an antibody and antigen, or a hormone and its receptor.

scribe the "on/off" elution mode often used in affinity chromatography to retain and detect the analyte of interest by placing the following statements in the correct order. In the image below, the circles represent the analyte of interest and the squares represent the remaining sample components. First step Last step The column and affinity ligand are regenerated prior to the next sample. Sample containing the analyte of interest is applied to the column with the application buffer Nonretained sample components are washed out of the column. The analyte is detected as it leaves the column. An elution buffer is applied to the column to release the analyte. affinity ligand selectively binds the analyte of interest

Explanation / Answer

1. Smaple containing the analyte of interest is applied to the column with the aplication buffer

2. The affinity ligand selectively binds the analyte of interest

3. Non retained sample components are washed out of the column.

4. An elution buffer is applied to the column to release the analyte

5.The analyte is detected as it leaves the colum

6. The column and affinity ligand are regenerated prior to the next sample

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