One of the techniques used to sequence DNA involved the addition of dideoxynucle

Question

One of the techniques used to sequence DNA involved the addition of dideoxynucleotides to DNA replication. Dideoxynucleotides are missing the OH group on the 3' end, which is where DNA polymerase attaches new nucleotides. If these dideoxynucleotides are added to a cell nucleus during the S phase, what would you expect to happen? DNA polymerase will be damaged and replication will be prevented. Both the legging strand and the leading strand will have okazaki fragments DNA replication will continue, since DNA polymers can continue to add nucleotides to the 5'end. DNA replication will proceed faster, since there is no OH to get on the way of DNA polymerase

Explanation / Answer

If the dideoxynucleotides are attached to a cell during the phase, the DNA replication will proceed faster, since there is no OH to get on the way of DNA polymerase as dideoxynucleotides do not have a hydroxyl (OH) group at the 3' position.

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