Academic Integrity: tutoring, explanations, and feedback — we don’t complete graded work or submit on a student’s behalf.

A / - Trying to make a flow chart for these bacteria and I\'m have trouble. I\'m

ID: 97624 • Letter: A

Question

A / -

Trying to make a flow chart for these bacteria and I'm have trouble. I'm not even sure if my test results are correct. It'd be much apreciated if someone could fill me in on the test results for these microbes (including checking what is already filled). It'd be even more appreciated if someone could actually do the flow chart since I have some drafts on paper but keep getting mixed up. Thanks in advance!!

MICROBE Gram rxn/ Catalase H2S Mannitol/ Lactose EMB MR / Indole Citrate Colony Shape Activity prod. High Salt ferm./gas VP Color (growth/ferm) Enterobacter aerogenes neg. rod + - + acid/gas + -/+ - + Escherichia coli neg. rod + - + acid/gas + +/- + - Proteus vulgaris neg rod + + - -/- + / - + - Salmonella typhimurium neg. rod + + - or wkA / - + / - - + Pseudomonas fluorescens neg. rod - - or wkA / - +/- - / - - + Bacillus cereus pos. rod + + / - - or wkA / - Bacillus subtilis pos. rod + + / + - or wkA / - Lactobacillus plantarum pos. rod - - / - - or wkA / - Micrococcus luteus pos. cpcci + + / - - or wkA / - Staphylococcus aureus pos. cocci A / - Staphylococcus epidermidis pos. cocci A / - Streptococcus faecalis pos cocci +/+ A / - Streptococcus lactis pos. cocci - / -

A / -

Explanation / Answer

Enterobacter aerogenes-Gram negative, rod shaped.

Catalase activity-Positive

Mannitol Fermentation- + Acid/Gas

Lactose fermentation- + Acid/Gas

Methyl red- Negative

Indole -negative

Citrate-positive

Eosin-methylene blue(EMB) - positive

Escherichia coli- Gram negative, rod shaped.

Catalase activity-Positive

Mannitol Fermentation- + Acid/Gas

Lactose fermentation- + Acid/Gas

Methyl red/VP- Positive,V-negative

Indole -positive

Citrate-Negative

Proteus vulgaris-Gram negative

catalase-POSITIVE

H2S-positive

mannitol-negative

Lactose-negative

MR/VP-VP-Negative,MR positive

Citrate-negative

Indole-positive

EMB-positive

colorless colonies

Salmonella typhimurium-Gram negative

catalase-positive

citrate-Negative

H2S positive

Indole -negative

Methyl red-positive, VP-negative

Lactose- negative

Mannotol-positive

COLONY-COLORLESS

Hydrogen Sulfide (H2S)- Negative