Exhibit 5A The following diagram shows the anode, cathode, and plH gradient of a
ID: 97766 • Letter: E
Question
Exhibit 5A The following diagram shows the anode, cathode, and plH gradient of an isoelectric focusing bed anode+ -cathode -6. Refer to Exhibit 5 A. A mixture of asp, asn, and arg is placed in the bed where the pH is 7, and the current i turned on. From left to right, which a. asp asn arg b. arg asn asp c. asn asp arg d. arg asp asrn t represents the final positions of the individual amino acids? -7. In all forms of chromatography one way of identifying eluted substances is by a. fluorescence spectroscopy b. comparison with standards c. radioactive labeling d. treating fractions with a reagent that will cause a color change 8, Differential centrifugation at low speeds (several thousand RPM) is a useful step when a. organelles have been lysed. b. enzymes of interest have different sizes c. cell membranes must be left intact. d. ribosomes need to be broken down. e. there are either organelles or debris to separate. 9. In isoelectric focusing gel electrophoresis a. particular care must be taken to ensure the same pli along the length of the gel b. there is a phI gradient that parallels the electric field gradient c. the electric current is allowed to fluctuate d. the electric circuits of the apparatus must be very well insulated 10. Ammonium sulfate is useful in protein purification because a. it contains nitrogen and sulfur, both of which occur in proteins b. it is sparingly soluble in water, causing proteins to co-precipitate with it c. very pure proteins are obtained when it is used d. it forms ion-dipole interactions with water, making proteins less soluble and more likely to precipitate 11. The purity of an enzyme at various stages of purification is best measured by a. total protein. b. total enzyme activity c. specific activity of the enzyme. d. percent recovery of the protein. e. percent recovery of the enzyme.Explanation / Answer
Answer:
7) Comparison with standards
8) There are either organelles or debris to separate
9) There is a pH gradient that parallels the electric field gradient
10) it forms ion-dipole interaction with water making protein less soluble and more likely to precipitate
11) Specific activity of the enzyme
12) Cyanogen bromide
13) Cation or anion exchange
14) An anionic substance
15) All of the above
16) The identities and relative amount of amino acids in a protein
17) Hydrophobic interactions
18) Nuclei mitochondria and ribosomes into three separate fractions
19) It is usually performed in a 96 well plates
20) The log of the MW of proteins
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